Research note

Micropropagation of Eucalyptus radiata ssp. radiata Using Explants from Mature and Coppice Material

Published in: South African Forestry Journal
Volume 162, issue 1, 1992 , pages: 43–47
DOI: 10.1080/00382167.1992.9630435
Author(s): S.H. ChangDepartment of Forestry, RSA, D., G.M. DonaldDepartment of Forestry, RSA, G. JacobsDepartment of Horticulture, RSA


Nodes from young field-grown coppice regrowth and branches of a mature tree of Eucalyptus radiata ssp. radiata were cultured on a MS medium containing BA and NAA. BA at 0,5 mg/ℓ and NAA at 0,01-0,1 mg/ℓ were most effective in promoting axillary shoot proliferation from both coppice and adult tissue. BA concentration of the medium was reduced (0,1 mg/ℓ) to induce axial growth in regenerated bud primordia. 50–70 shoots could be obtained from one nodal culture. Multiplication rate was higher from the mature tree than from the coppice shoots. Rooting was achieved from shoot cultures on half strength MS salts supplemented with 2,5 mg/ℓ of IBA for mature shoots and 5 mg/ℓ for coppice shoots. The percentage of rooting of coppice shoots (90%) was much higher than for mature shoots (35%). Summer was the best season to take explant material for shoot multiplication.

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