Short communications

Qualitative estimation of atrazine, hydroxyatrazine and 6-methoxy-2-benzoxazinone (MBOA) in maize plants

Published in: South African Journal of Plant and Soil
Volume 3 , issue 2 , 1986 , pages: 82–84

DOI: 10.1080/02571862.1986.10634195

Author(s): C. Malan , Republic of South Africa , J.H. Visser Department of Botany, Republic of South Africa , H.A. van de Venter Department of Botany, Republic of South Africa

Keywords: Atrazine , benzoxazinone , HPLC , hydroxy-atrazine , maize

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Abstract

A rapid and simple method for the extraction and quantification of the herbicide atrazine, the atrazine hydroxylating agent in maize plants DIMBOA, and the hydroxylated product hydroxy-atrazine, is described. These substances were quantified by means of HPLC techniques, employing a RP-18 column and a mobile phase of methanol:water (40:60), buffered at pH 7,4. This method was developed to determine the efficiency of the atrazine hydroxylation detoxifying mechanism in maize plants cultivated under different environmental stress conditions.

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