Research Papers

Enzymatic activities as potential stress biomarkers of two substituted benzene compounds in Propsilocerus akamusi (Diptera: Chironomidae)

Published in: African Journal of Aquatic Science
Volume 37, issue 3, 2012 , pages: 265–270
DOI: 10.2989/16085914.2012.703127
Author(s): CW CaoDepartment of Forest Protection, People's Republic of China, XP LiDepartment of Forest Protection, People's Republic of China, SL GeDepartment of Forest Protection, People's Republic of China, LL SunDepartment of Forest Protection, People's Republic of China, ZY WangDepartment of Forest Protection, People's Republic of China

Abstract

Chironomids are a globally distributed family of insects that can serve as biological indicators of environmental pollution. Substituted benzenes are a group of serious environmental pollutants and severely threaten biological and human health. In order to investigate potential stress biomarkers of substituted benzenes, the effects of 4-chlorophenol and p-phenylene diamine on superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), carboxylesterase (CarE), acid phosphatase (ACP) and alkaline phosphatase (ALP) activities of 4th-instar larval Propsilocerus akamusi were investigated. During 48 h exposure to 4-chlorophenol and p-phenylene diamine, the larval CAT, SOD, GST and CarE activities responded to 4-chlorophenol and p-phenylene diamine in a concentration-and time-dependent way. The ACP and ALP activities of larval P. akamusi responded slowly to 4-chlorophenol and p-phenylene diamine, and only the ALP activities were inhibited significantly under the exposure to p-phenylene diamine for 6 and 24 h. These results suggest that CAT, SOD, GST and CarE activities of chironomids can be used as potential stress biomarkers to monitor 4-chlorophenol and p-phenylene diamine pollution.

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