Search
Search results for
We found
16 results for you
-
Barley cultivar identification by acid Polyacrylamide gel electrophoresis of hordein proteins
Item type: Journal Article • Journal: South African Journal of Plant and Soil • Authors: O.T. de Villiers --- Department of Agronomy and Pastures, Republic of South Africa E.W. Laubscher --- Department of Agronomy and Pastures, Republic of South AfricaMethods are described for the extraction and separation of hordein proteins of barley by acid Polyacrylamide gel electrophoresis as a means of identifying different cultivars. Experimental conditions for the extraction of the proteins from single kernels and from corresponding flour... -
Drought-related protein synthesis in cotton
Item type: Journal Article • Journal: South African Journal of Plant and Soil • Authors: A. van der Mescht --- , Republic of South Africa J.A. de Ronde --- , Republic of South AfricaThe changes in protein profiles of six cotton cultivars subjected to osmotic stress were examined. Osmotic stress was applied by incubating leaf segments in mannitol. The efficiency of protein synthesis decreased with increasing concentrations of mannitol. Drought-related protein synthesis during... -
Wheat cultivar identification by electrophoretic analysis of gliadin proteins
Item type: Journal Article • Journal: South African Journal of Plant and Soil • Authors: O.T. de Villiers --- Department of Botany, Republic of South Africa M. Bosman --- Centre for Cereal Research, Department of Agronomy and Pastures, Republic of South AfricaPolyacrylamide gel electrophoresis of gliadin proteins in an acid medium was used to identify different spring wheat cultivars. Gliadins were extracted from single kernels or corresponding flour samples using different solvents. Gliadin electrophoretogram formulae using relative band mobilities and staining... -
Identification of tall fescue grass cultivars by sodium dodecyl sulphate and acid polyacrylamide gel electrophoresis
Item type: Journal Article • Journal: South African Journal of Plant and Soil • Authors: R.E. Hierse --- , Republic of South Africa O.T. de Villiers --- Department of Botany, Republic of South Africa M. Bosman --- Department of Botany, Republic of South AfricaProtein banding patterns of seed obtained by sodium dodecyl sulphate Polyacrylamide gel electrophoresis (SDS-PAGE) or acid Polyacrylamide gel electrophoresis (A-PAGE) were used to distinguish between different tall fescue (Festuca arundinacea Shreb.) grass cultivars. An electrophoretic formula was determined for each... -
Extraction and electrophoretic separation of proteins from leaves of scions and rootstocks of Vitis cultivars
Item type: Journal Article • Journal: South African Journal of Plant and Soil • Authors: E.W. le Grange --- , Republic of South Africa O.T. de Villiers --- Department of Botany, Republic of South AfricaProteins were extracted and separated by SDS-PAGE from leaves of scions and rootstocks of different Vitis cultivars. Banding patterns were used to identify the different cultivars after extraction of the proteins with a medium containing Tris-HCI (0.68 M, pH 7.8),... -
Identification of Cyclopia species (‘Heuningbostee’) using Polyacrylamide gel electrophoresis
Item type: Journal Article • Journal: South African Journal of Plant and Soil • Authors: O.T. de Villiers --- Department of Botany, South Africa M. Bosman --- Department of Botany, South Africa J.H. de Lange --- , South AfricaProtein banding patterns of seed obtained by sodium dodecyl sulphate Polyacrylamide get electrophoresis (SDS-PAGE) or acid polycrylamide gel electrophoresis (A-PAGE) were used to distinguish between different Cyclopia species (‘heuningbostee’). An electrophoretic formula was determined for each species using relative band... -
The use of acid Polyacrylamide gel electrophoresis to determine the effect of locality of growth on the protein profiles of barley, wheat and honeybush tea
Item type: Journal Article • Journal: South African Journal of Plant and Soil • Authors: O.T. de Villiers --- Department of Botany, South Africa M. Bosman --- Centre for Cereal Research, Department of Agronomy and Pastures, South AfricaAcid Polyacrylamide gel electrophoresis (A-PAGE) of proteins was used to identify different spring wheat cultivare, barley cultivars and Cyclopia species. Experimental conditions for the extraction of the proteins from single kernels as well as corresponding flour samples are described. Different... -
Electrophoresis of leaf and seed proteins of Englerophytum natalense (fam. Sapotaceae)
Item type: Journal Article • Journal: South African Journal of Plant and Soil • Authors: H. Ham --- Department of Forestry, South Africa M. Bosman --- , South Africa P.J. Vorster --- Department of Botany, South AfricaEnglerophytum natalense is an indigenous South African tree, bearing edible fruits. In view of its potential for domestication, leaf and seed proteins were separated electrophoretically in order to investigate the existence and degree of variation within and between populations in... -
THE PLASMA PROTEINS OF SOME FRESHWATER FISH IN THEIR NATURAL ENVIRONMENT
Item type: Journal Article • Journal: Journal of the Limnological Society of Southern Africa • Authors: J. H.J. van Vuren --- Division of Nature Conservation, South AfricaThe plasma proteins of Cyprinus carpio (carp), Barbus holubi (yellowfish), Labeo umbratus (mudfish) and Labeo capensis (mudfish) were electrophoretically separated on polyacrylamide gels from blood samples obtained from specimens sampled during summer and winter in three different localities. Within a... -
Biochemical genetic variation between four populations of Labeobarbus polylepis from three river systems in South Africa
Item type: Journal Article • Journal: African Journal of Aquatic Science • Authors: PFS Mulder D Engelbrecht JS Engelbrecht F RouxThe genetic variation within four Labeobarbus polylepis populations from both river and dam environments in the Limpopo, Incomati and Phongolo River systems was studied. Gene products of 22 enzyme-coding loci were resolved using horizontal starch gel electrophoresis. Fourteen (64%) of... -
Population genetic structure of the sidespot barb, Barbus neefi, from the north-eastern escarpment, South Africa
Item type: Journal Article • Journal: African Journal of Aquatic Science • Authors: GD Engelbrecht --- Discipline of Physiology, South Africa P FS Mulder --- Discipline of Physiology, South Africa JS Engelbrecht --- , South AfricaAllozyme analysis was used to determine patterns of genetic variation within and between populations of Barbus neefi. The products of 29 loci were analysed, with 17 loci being monomorphic in all populations. The genetic variability estimates compared well with values... -
Large allozyme variation within populations and isozyme differences between Clarias gariepinus and C. ngamensis from the Okavango Delta, Botswana
Item type: Journal Article • Journal: African Journal of Aquatic Science • Authors: Herman van der Bank Nico SmitAn allozyme study of 47 Clarias gariepinus and 53 C. ngamensis individuals revealed variation at 11 (57.9%) and eight (40%), out of 19 and 20 loci, respectively. The average heterozygosity of 0.167, 57.9% polymorphism and average of 1.895 alleles at... -
Low allozyme variation in tigerfish Hydrocynus vittatus (Teleostei: Alestidae) from the Okavango panhandle, with notes on the selection of candidates for artificial breeding
Item type: Journal Article • Journal: African Journal of Aquatic Science • Authors: M Soekoe --- Centre for Aquatic Research, Department of Zoology, South Africa NJ Smit --- Centre for Aquatic Research, Department of Zoology, South Africa H van der Bank --- Centre for Aquatic Research, Department of Zoology, South AfricaTo ensure the utilisation of optimal tigerfish gene pools for artificial propagation, the hypothesis that tigerfish from the Okavango system should have greater heterozygosity than those from smaller river systems was tested. This electrophoretic analysis provides the first report of... -
Phenotypic plasticity and local adaptation in two extreme populations of Acacia karroo
Item type: Journal Article • Journal: African Journal of Range & Forage Science • Authors: G Bayonne Mboumba D WardPhenotypic plasticity is the effect of growth variability in response to different environmental conditions. Adaptive phenotypic plasticity is more specific; it is the ability of a single genotype to produce an array of phenotypes depending on the environmental extent. We... -
Genetic variability of South African fiscal shrikes (Lanius collaris) across an altitudinal gradient
Item type: Journal Article • Journal: African Zoology • Authors: S. Soobramoney --- School of Biological & Conservation Sciences, University of KwaZulu-Natal, South Africa G.K. Campbell --- School of Life and Environmental Sciences, University of KwaZulu-Natal, South Africa C.T. Downs --- School of Biological & Conservation Sciences, University of KwaZulu-Natal, South Africa N.J. Adams --- School of Biological & Conservation Sciences, University of KwaZulu-Natal, South AfricaAn electrophoretic allozyme study of the fiscal shrike Lanius collaris, a widely distributed sub-Saharan passerine, was undertaken to investigate altitudinal variation in genetic structure within this species. It is a sedentary bird with limited dispersal so that subpopulations may be... -
Patterns of genetic heterogeneity in Neutraclinostomum intermedialis (Digenea: Clinostomatidae): geographical and temporal considerations
Item type: Journal Article • Journal: African Zoology • Authors: J.P Grobler [AN0001] N.M. Mokgalong --- School of Molecular and Life Sciences, South AfricaApproximately 300 individuals of Neutraclinostomum intermedialis from three localities in the Northern Province of South Africa were screened using allozyme electrophoresis. The aim was to determine genetic diversity and divergence within and between populations. The within population heterozygosity values (H...