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A comparison of HMW-GS, LMW-GS and RAPD fingerprinting for cultivar identification of some South African wheat cultivars
Item type: Journal Article • Journal: South African Journal of Plant and Soil • Authors: M.T. Labuschagne --- Department of Plant Breeding, Republic of South Africa H. Maartens --- Department of Plant Breeding, Republic of South Africa A.M. Oberholster --- Department of Genetics, Republic of South AfricaAccurate identification of breeding material is very important for the plant breeder. In South Africa, high molecular weight glutenin subunits (HMW-GS) have so far been used for wheat cultivar identification. This method can often not distinguish between cultivars, and other... -
RAPD analysis of selected common bean (Phaseolus vulgaris L.) cultivars from three African countries
Item type: Journal Article • Journal: South African Journal of Plant and Soil • Authors: C. M.S. Mienie --- , Republic of South Africa L. Herselman --- , Republic of South Africa R.E. Terhlanche --- , Republic of South AfricaDNA from six dry bean cultivars was analysed with a total of thirty two primers in RAPD analysis to develop a method to distinguish between the different cultivars. The varieties are cultivated in three African countries and belong to two... -
Development of a sequence characterized amplified region (SCAR) marker for the identification of the potato cultivars Astrid and Mnandi
Item type: Journal Article • Journal: South African Journal of Plant and Soil • Authors: W.S. Jansen van Rensburg --- , South Africa I.A. Dubery --- Department of Biochemistry, South AfricaAstrid and Mnandi are two closely related potato cultivars of commercial importance in Southern Africa. Random amplified polymorphic DNA (RAPD) markers were screened and a sequence characterized amplified region (SCAR) marker was developed to distinguish between these two cultivars since... -
Development of SCAR markers for a Thinopyrum distichum chromosome that appears to be involved in salt tolerance
Item type: Journal Article • Journal: South African Journal of Plant and Soil • Authors: J.A. Jacobs --- Department of Genetics, South Africa L. Hanekom --- Department of Genetics, South Africa A.S. Marais --- Department of Genetics, South Africa G.F. Marais --- Department of Genetics, South AfricaSixty-seven F-1 plants from the backcross: Thinopyrum distichum/tetraploid ‘Henoch’ rye//diploid ‘Henoch’ rye were cloned, tested for salt tolerance (2–4 replications per genotype) and ranked. Sixty RAPD primers were tested on the parental lines. Nine of these produced Thinopyrum specific fragments... -
Low genetic diversity and high genetic differentiation among severely fragmented populations of the critically endangered tree Talbotiella gentii (Fabaceae)
Item type: Journal Article • Journal: Southern Forests: a Journal of Forest Science • Authors: D Dompreh --- School of Biological Sciences, UK MD Swaine --- School of Biological Sciences, UK A Price --- School of Biological Sciences, UKThe genetic diversity among 17 populations (including 10 newly discovered) of Talbotiella gentii (Fabaceae, Caesalpinioideae), a rare tree species endemic to Ghana, was assessed using random amplified polymorphic DNA (RAPD). A total of 80 polymorphic bands were generated from eight... -
RAPD markers demonstrate genetic diversity in Pterocarpus angolensis from Zimbabwe and Zambia
Item type: Journal Article • Journal: Southern Forests: a Journal of Forest Science • Authors: E Chisha-Kasumu S Woodward A PriceUnderstanding the availability, extent and apportionment of genetic variability in natural populations of the southern African savanna tree Pterocarpus angolensis can provide reliable information on the evolutionary history and phylogenetic relationships of P. angolensis populations. This information will aid strategies...