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Synthesis of heat-shock proteins in six cotton cultivars at different heat treatments
Item type: Journal Article • Journal: South African Journal of Plant and Soil • Authors: J.A. de Ronde --- , Republic of South Africa W.A. Cress --- Department of Botany, Republic of South AfricaChanges in protein synthesis in leaf tissue of six cotton cultivars were studied by comparing the heat-shock protein patterns synthesized at different heat treatments. Leaf tissue responded to thermotolerance treatments by synthesizing a specific set of proteins for each treatment... -
Extraction and electrophoretic separation of proteins from leaves of scions and rootstocks of Vitis cultivars
Item type: Journal Article • Journal: South African Journal of Plant and Soil • Authors: E.W. le Grange --- , Republic of South Africa O.T. de Villiers --- Department of Botany, Republic of South AfricaProteins were extracted and separated by SDS-PAGE from leaves of scions and rootstocks of different Vitis cultivars. Banding patterns were used to identify the different cultivars after extraction of the proteins with a medium containing Tris-HCI (0.68 M, pH 7.8),... -
The use of size exclusion high performance liquid chromatography (SE-HPLC) for wheat quality prediction in South Africa
Item type: Journal Article • Journal: South African Journal of Plant and Soil • Authors: M.T. Labuschagne --- Department of Plant Sciences, South Africa U. Aucamp --- , South AfricaSDS-PAGE and RP-HPLC imply a total reduction of native protein aggregates. This leads to a loss of information concerning structure, interaction and stability of protein complexes. A major advantage of SE-HPLC is its potential to work with relatively large aggregates,... -
Persistence of the pesticidal Bacillus thuringiensis protein expressed in Bt maize plant materials in two soils of the Central Eastern Cape, South Africa
Item type: Journal Article • Journal: South African Journal of Plant and Soil • Authors: P. Muchaonyerwa --- Department of Agronomy, South Africa S.M. Waladde --- Department of Zoology, South AfricaEnvironmental effects of genetically modified plants are not yet fully understood. Experiments were conducted to determine relative amounts of the bioactive Bt protein in roots, leaves and stems of Bt maize and persistence of the protein in two soil forms... -
THE PLASMA PROTEINS OF SOME FRESHWATER FISH IN THEIR NATURAL ENVIRONMENT
Item type: Journal Article • Journal: Journal of the Limnological Society of Southern Africa • Authors: J. H.J. van Vuren --- Division of Nature Conservation, South AfricaThe plasma proteins of Cyprinus carpio (carp), Barbus holubi (yellowfish), Labeo umbratus (mudfish) and Labeo capensis (mudfish) were electrophoretically separated on polyacrylamide gels from blood samples obtained from specimens sampled during summer and winter in three different localities. Within a... -
Accumulation of hepatic Hsp70 and plasma cortisol in Oreochromis mossambicus following sublethal metal and DDT exposure
Item type: Journal Article • Journal: African Journal of Aquatic Science • Authors: SS Mlambo --- Centre for Aquatic Research, Zoology Department, South Africa JHJ van Vuren --- Centre for Aquatic Research, Zoology Department, South Africa R Basson --- Centre for Aquatic Research, Zoology Department, South Africa B Grant --- Centre for Aquatic Research, Zoology Department, South AfricaHeat shock response in Oreochromis mossambicus livers was measured, using antibodies specific to Hsp70 family members, by an immunoblotting protocol. The hepatic isoforms Hsp70, Hsp74 and Hsp76 were identified and quantified from copper exposures. Long-term DDT exposure did not result... -
Alterations in serum protein fractions and Na+-K+ATPase activity in Clarias gariepinus infested with henneguyosis in Ismailia, Egypt
Item type: Journal Article • Journal: African Journal of Aquatic Science • Authors: DM Sabri MA Danasoury IAM Eissa HM KhouraibaTotal serum protein, protein fractions and Na+-K+ATPase activity were estimated in Clarias gariepinus collected from surface waters in Ismailia governorate, Egypt. Comparisons were made between fish infested with respiratory henneguyosis and non-infested fish. Results of serum analysis revealed a significant... -
Carotid intima-media thickness and its associations with type 2 diabetes mellitus in South Africans
Item type: Journal Article • Journal: Journal of Endocrinology, Metabolism and Diabetes of South Africa • Authors: P Rheeder --- Epidemiology and Biostatistics Track, School of Health Systems and Public Health, University of Pretoria, MC Duim-Beytell --- School of Medicine, University of Pretoria, R Meijer --- Julius Centre for Health Sciences and Primary Care, Netherlands T Gustavsson --- Image and Data Analysis, Sweden GKG Danler --- Department of Radiology, RA Ahmed --- Department of Radiology, DG Van Zyl --- Department of Internal Medicine,Objectives: Carotid intima-media thickness (CIMT) is a surrogate marker of subclinical atherosclerosis and a predictor of cardiovascular events. Few studies in Africa have evaluated CIMT and its associations in people with type 2 diabetes mellitus. This study measured CIMT in... -
The channelopathies: an overview
Item type: Journal Article • Journal: Southern African Journal of Anaesthesia and Analgesia • Authors: Joanne M Blanckenberg [AN0001]What at first glance appears to be a random selection of widely differing clinical presentations and syndromes, has recently been found to have as their common underlying factor an inherited abnormality of the mechanism in the cell wall, the ion... -
The metabolic profiles of HIV-infected and non-infected women in Mangaung, South Africa
Item type: Journal Article • Journal: South African Journal of Clinical Nutrition • Authors: Z. Hattingh --- Department of Nutrition and Dietetics, C. Walsh --- Department of Nutrition and Dietetics, Fj Veldman --- Fibrinogen Unit, Cj Bester --- Department of Biostatistics,Objective: To determine the biochemical nutritional status of HIV-infected women in Mangaung. -
The role of proline and root traits on selection for drought-stress tolerance in soybeans: a review
Item type: Journal Article • Journal: South African Journal of Plant and Soil • Authors: Obed J Mwenye --- Department of Plant Sciences, South Africa Leon van Rensburg --- Department of Soil, Crop and Climate Sciences, South Africa Angeline van Biljon --- Department of Plant Sciences, South Africa Rouxléne van der Merwe --- Department of Plant Sciences, South AfricaDrought stress is a major limiting factor in soybean production. It reduces both the yield and its components. This is dependent on the level and occurrence of drought stress with respect to the growth stages of the plants. Breeding for... -
The effect of low-level kelp supplementation on digestive enzyme activity levels in cultured abalone Haliotis midae fed formulated feeds
Item type: Journal Article • Journal: African Journal of Marine Science • Authors: A Nel --- Department of Ichthyology and Fisheries Science, South Africa BI Pletschke --- Department of Biochemistry and Microbiology, South Africa PJ Britz --- Department of Ichthyology and Fisheries Science, South AfricaPrevious studies have found that macroalgal inclusion in formulated diets for cultured abalone improves feed utilisation, and it was suggested that seaweed-associated bacteria supply enzymes that aid in the animal's digestion. In the present study, we hypothesised that kelp supplementation... -
Maize grain soluble sugar and protein contents in response to simulated hail damage
Item type: Journal Article • Journal: South African Journal of Plant and Soil • Authors: Silindile P Miya --- Crop Science, School of Agricultural, Earth and Environmental Sciences, South Africa Albert T Modi --- Crop Science, School of Agricultural, Earth and Environmental Sciences, South Africa Samson Z Tesfay --- Crop Science, School of Agricultural, Earth and Environmental Sciences, South Africa Tafadzwanashe Mabhaudhi --- Crop Science, School of Agricultural, Earth and Environmental Sciences, South AfricaProduction of grain of high nutritional quality is essential in maize (Zea mays L.) as a food grain crop. Grain quality may be influenced by environmental and management factors, such as hail, plant density and grain selection. A study was...