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Microsatellite markers for population genetic studies of the rock firefinch, Lagonosticta sanguinodorsalis
Item type: Journal Article • Journal: African Zoology • Authors: Jacinta Abalaka --- Department of Genetics, South Africa Bengt Hansson --- Department of Biology, SwedenWe identified the first set of microsatellites for use in the rock firefinch (Lagonosticta sanguinodorsalis). First, we tested existing passerine microsatellite loci for cross-species amplification success in a small sample of rock firefinches and identified 10 loci that were seemingly... -
Genomic resources for the spotted ragged-tooth shark Carcharias taurus
Item type: Journal Article • Journal: African Journal of Marine Science • Authors: JD Klein --- Centre for Ecological Genomics and Wildlife Conservation, Department of Zoology, South Africa AE Bester-van der Merwe --- Molecular Breeding and Biodiversity Group, Department of Genetics, South Africa ML Dicken --- KwaZulu-Natal Sharks Board, South Africa A Emami-Khoyi --- Centre for Ecological Genomics and Wildlife Conservation, Department of Zoology, South Africa KL Mmonwa --- KwaZulu-Natal Sharks Board, South Africa PR Teske --- Centre for Ecological Genomics and Wildlife Conservation, Department of Zoology, South AfricaGenomic data can be a useful tool in the management and conservation of biodiversity. Here, we report the development of genomic resources for the spotted ragged-tooth shark Carcharias taurus using genome-wide DNA data from Illumina next-generation sequencing. We explored two... -
A pilot study on the use of DNA metabarcoding for diet analysis in a montane amphibian population from North Africa
Item type: Journal Article • Journal: African Journal of Herpetology • Authors: Ana Pereira --- Universidade do Porto, Portugal Mohamed Amine Samlali --- Cadi Ayyad University, Morocco Abderrahim S’Khifa --- Cadi Ayyad University, Morocco Tahar Slimani --- Cadi Ayyad University, Morocco D James Harris --- Universidade do Porto, PortugalAlthough dietary studies are essential to design effective conservation strategies for amphibians, non-invasive studies using microscopy assessment of faecal material are known to have limitations. We assessed the use of DNA metabarcoding to determine diet in the Moroccan painted frog... -
An allele-specific PCR assay for detection of mutations that confer benzimidazole resistance and zoxamide sensitivity in Phyllosticta citricarpa
Item type: Journal Article • Journal: South African Journal of Plant and Soil • Authors: Beatrix Coetzee --- Stellenbosch University, South Africa Elma Carstens --- Citrus Research International (Pty) Ltd, South Africa Gerhardus C Schutte --- Avima (Pty) Ltd, South Africa Elaine Basson --- Citrus Research International (Pty) Ltd, South Africa Mia J Groeneveld --- Stellenbosch University, South Africa Tankiso Mpholo --- Land Reform and Rural Development, South Africa Providence Moyo --- Citrus Research International (Pty) Ltd, South Africa Jessica C Winn --- Stellenbosch University, South Africa Paul H Fourie --- Citrus Research International (Pty) Ltd, South Africa Aletta E Bester van der Merwe --- Stellenbosch University, South AfricaPhyllosticta citricarpa is the causative agent of citrus black spot (CBS), a cosmetic fungal disease that has been reported in most of the citrus-growing regions of the world. The occurrence of CBS in orchards is predominantly controlled by the application...