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Culture-independent detection and quantification of Fusarium circinatum in a pine-producing seedling nursery
Item type: Journal Article • Journal: Southern Forests: a Journal of Forest Science • Authors: Gerda Fourie --- Department of Microbiology and Plant Pathology, South Africa Michael J Wingfield --- Department of Microbiology and Plant Pathology, South Africa Brenda D Wingfield --- Department of Genetics, Forestry and Agricultural Biotechnology Institute, South Africa Nicky B Jones --- Sappi Forests, Shaw Research Centre, South Africa Andrew R Morris --- Sappi Forests, Shaw Research Centre, South Africa Emma T Steenkamp --- Department of Microbiology and Plant Pathology, South AfricaThe primary symptoms associated with Fusarium circinatum infection in pine seedling nurseries are root and collar rot, shoot and tip die-back and seedling mortality. Management of this pathogen in nurseries usually involves the integration of various strategies relating to sanitation,... -
Prevalence of Clostridium difficile toxin genes in Pretoria
Item type: Journal Article • Journal: Southern African Journal of Epidemiology and Infection • Authors: N Storm --- Department of Medical Microbiology, M M Kock --- Department of Medical Microbiology, M D Kruger --- Department of Medical Microbiology, M M Ehlers --- Department of Medical Microbiology,The hypervirulent polymerase chain reaction (PCR) ribotype 027 strain of Clostridium diffcile produces toxins A, B and a binary toxin. Toxin detection kits are commonly used in diagnostic laboratories, but have been unsuccessful in detecting all of the relevant C... -
Current status of urinary schistosomiasis in communities around the Erinle and Eko-Ende Dams and the implications for schistosomiasis control in Nigeria
Item type: Journal Article • Journal: Southern African Journal of Infectious Diseases • Authors: A O Hassan --- Department of Medical Microbiology and Parasitology Lautech Teaching Hospital, Nigeria A O J Amoo --- Medical Mecrobiology and Parasitology Department, Nigeria O P Akinwale --- Molecular Parasitology Research Laboratory, Public Health Division, Nigerian Institute of Medical Research, Nigeria A M Deji-Agboola --- Medical Mecrobiology and Parasitology Department, Nigeria M A Adeleke --- Public Health Entomology and Parasitology Unit, Nigeria P V Gyang --- Molecular Parasitology Research Laboratory, Public Health Division, Nigerian Institute of Medical Research, NigeriaSchistosomiasis is an endemic disease in many parts of the world and the disease is associated with water resource development projects, such as dams, irrigation schemes, and rice and fish farming. The present study was designed to determine the prevalence... -
A PCR-based method for sex identification in Hippopotamus amphibius
Item type: Journal Article • Journal: African Zoology • Authors: Richard Beckwitt --- Department of Biology, Framingham State College, U.S.A. Jennifer Shea --- Department of Biology, Framingham State College, U.S.A. Deirdre Osborne --- Department of Biology, Framingham State College, U.S.A. Stephen Krueger --- The Toledo Zoo, U.S.A. William Barklow --- Department of Biology, Framingham State College, U.S.A.Identification of the sex of aquatic mammals such as the hippopotamus (Hippopotamus amphibius) is difficult in the field. We have developed a PCR-based method for sex identification in the hippopotamus. This method amplifies a short fragment of the ZFX and... -
Diversity and identification of fungi associated with needles of Pinus radiata in Tasmania
Item type: Journal Article • Journal: Southern Forests: a Journal of Forest Science • Authors: Istiana Prihatini --- Tasmanian Institute of Agriculture, Australia Morag Glen --- Tasmanian Institute of Agriculture, Australia Tim J Wardlaw --- Forestry Tasmania, Hobart, Australia Caroline L Mohammed --- Tasmanian Institute of Agriculture, AustraliaNeedle fungi have been extensively studied in conifers but rarely in Pinus radiata. Previous studies of P. radiata have been based on fungal isolation and not direct PCR detection from needles. This research was a component of a study examining... -
Testing for the occurrence of pilchard herpesvirus (PHV) in South African sardine Sardinops sagax
Item type: Journal Article • Journal: African Journal of Marine Science • Authors: BM Macey --- Branch: Fisheries Management, Department of Agriculture, South Africa KW Christison --- Branch: Fisheries Management, Department of Agriculture, South Africa J de Goede --- Branch: Fisheries Management, Department of Agriculture, South Africa L Hutchings --- Marine Research Institute and Department of Biological Sciences, South Africa CD van der Lingen --- Branch: Fisheries Management, Department of Agriculture, South AfricaCatches of South African sardine Sardinops sagax have declined in recent years from about 200 000 t harvested annually during the period 2002–2006 to less than 100 000 t. Consequently, some companies are now importing sardine from sources elsewhere in... -
Multiplex-ready PCR assay of SSR marker diversity among quality protein maize inbred parental lines
Item type: Journal Article • Journal: South African Journal of Plant and Soil • Authors: Adeniyi S Atanda --- Department of Agronomy, Nigeria Gbadebo Olaoye --- Department of Agronomy, NigeriaPhenotypic markers have long been used for classification of maize (Zea mays) cultivars. However, because of shortcomings in detecting differences among closely related genotypes, coupled with sensitivity to environmental conditions, molecular marker techniques have been employed in the determination of... -
Evaluation of the prevention of mother-to-child transmission programme at a primary health care centre in South Africa
Item type: Journal Article • Journal: South African Family Practice • Authors: OS Akinsanya --- Department of Public Health, UK JA Wiseberg-Firtell --- Department of Public Health, UK G Akpomiemie --- Reproductive Health & HIV Institute, University of the Witswatersrand, South Africa OV Adeniyi --- Department of Family Medicine, South Africa RP Kaswa --- Department of Family Medicine, South AfricaAim: To evaluate the effectiveness of the prevention of mother-to-child transmission (PMTCT) of the HIV programme at Levai Mbatha Community Health Centre (CHC), Evaton, South Africa. -
Development of gene expression assays measuring immune responses in the spotted hyena (Crocuta crocuta)
Item type: Journal Article • Journal: African Zoology • Authors: Roxanne L Higgitt --- DST/NRF Centre of Excellence for Biomedical Tuberculosis Research/SAMRC Centre for TB Research/Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, South Africa Peter E Buss --- Veterinary Wildlife Services, South African National Parks, Kruger National Park, South Africa Paul D van Helden --- DST/NRF Centre of Excellence for Biomedical Tuberculosis Research/SAMRC Centre for TB Research/Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, South Africa Michele A Miller --- DST/NRF Centre of Excellence for Biomedical Tuberculosis Research/SAMRC Centre for TB Research/Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, South Africa Sven DC Parsons --- DST/NRF Centre of Excellence for Biomedical Tuberculosis Research/SAMRC Centre for TB Research/Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, South AfricaAs scavengers, spotted hyenas (Crocuta crocuta) are exposed to a wide array of pathogens but exhibit low mortality rates due to infectious disease. This suggests that this species exhibits a unique and robust immune response to pathogens. However, few tools... -
The recovery and molecular identification of HAdV-D17 in raw sewage and mussel samples collected in the Eastern Cape province of South Africa
Item type: Journal Article • Journal: Southern African Journal of Infectious Diseases • Authors: Hillary J Vos --- Department of Biochemistry and Microbiology, South Africa Caroline M Knox --- Department of Biochemistry and Microbiology, South AfricaHuman adenoviruses (HAdV) are a common cause of clinical infections in South Africa. However, there is a lack of information regarding the prevalence and molecular identification of this virus in the environment. The objective of this study was to investigate... -
The impact of cerebrospinal fluid viral analysis on empiric antibiotic use in children admitted to Tygerberg Children’s hospital with suspected meningitis
Item type: Journal Article • Journal: Southern African Journal of Infectious Diseases • Authors: Irma Kruger --- Department of Paediatrics and Child Health, South Africa Jean Maritz --- PathCare Reference Laboratory, South Africa Heather Finlayson --- Department of Paediatrics and Child Health, South AfricaBackground: Viral meningitis is the most common form of aseptic meningitis and requires minimal investigation and treatment. Polymerase chain reaction (PCR) has become the ‘gold standard’ for identifying viruses in cerebrospinal fluid and can provide rapid results. The objective of... -
Prevalence of Kudoa thyrsites (Myxozoa) in South African sardine Sardinops sagax, kingklip Genypterus capensis, and Cape hakes Merluccius capensis and M. paradoxus
Item type: Journal Article • Journal: African Journal of Marine Science • Authors: SS Henning --- Department of Food Science and Technology, South Africa M Krügel --- Department of Food Science, South Africa M Manley --- Department of Food Science, South AfricaPrevalence of infection with the myxozoan parasite Kudoa thyrsites is not well documented in sardine Sardinops sagax, the Cape hakes Merluccius capensis and M. paradoxus, and kingklip Genypterus capensis. Quantitative polymerase chain reaction assays were used to determine the prevalence... -
First report of Wolbachia from field populations of Culex mosquitoes in south-western Nigeria
Item type: Journal Article • Journal: African Zoology • Authors: Tosin Segun Ogunbiyi --- Redeemer’s University, Nigeria Philomena Eromon --- Redeemer’s University, Nigeria Paul Oluniyi --- Redeemer’s University, Nigeria Femi Ayoade --- Redeemer’s University, Nigeria Owoicho Oloche --- Redeemer’s University, Nigeria Judith Uche Oguzie --- Redeemer’s University, Nigeria Onikepe Folarin --- Redeemer’s University, Nigeria Christian Happi --- Redeemer’s University, Nigeria Isaac Komolafe --- Redeemer’s University, NigeriaRecent reports on finding Wolbachia-strain infections in field mosquito species in some West African countries and the potential for developing these as disease vector biocontrol tools have prompted a search for Wolbachia in mosquitoes within the study area. Using a... -
BRAF testing in a South African cohort of MLH1 deficient endometrial carcinomas: lessons learnt
Item type: Journal Article • Journal: Southern African Journal of Gynaecological Oncology • Authors: Reubina Wadee --- , South Africa Wayne Grayson --- , South Africa -
An enigmatic mortality event in the only population of the Critically Endangered Cameroonian frog Xenopus longipes
Item type: Journal Article • Journal: African Journal of Herpetology • Authors: DavidC. Blackburn --- University of Kansas, USA BenJ. Evans --- McMaster University, Canada AllanP. Pessier --- San Diego Zoo's Institute for Conservation Research, USA VanceT. Vredenburg --- San Francisco State University, USAContemporary global declines and mortality events in amphibian populations have been often attributed to infectious disease and climate change, separately and in combination. We report on an enigmatic mortality event in the only known population of the Critically Endangered frog...