Articles by Keyword: Molecular Identification

Search results for Molecular Identification

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  1. Molecular research on the systematically challenging smoothhound shark genus <em>Mustelus</em>: a synthesis of the past 30 years

    Molecular research on the systematically challenging smoothhound shark genus <em>Mustelus</em>: a synthesis of the past 30 years

    Item type: Journal Article • Journal: African Journal of Marine Science
    The species-rich genus Mustelus (smoothhounds) of the shark family Triakidae is one of the most bio-economically important groups of elasmobranchs in the world’s oceans. Despite the commercial value of Mustelus, the systematics of the group remains largely unresolved and there...
  2. The occurrence of mycotoxigenic fungi in abalone feed in South Africa

    The occurrence of mycotoxigenic fungi in abalone feed in South Africa

    Item type: Journal Article • Journal: African Journal of Marine Science
    Abalone feed in South Africa is mainly produced from locally sourced grains and has soy or fishmeal as a protein source. Contamination with fungal species associated with grain-based animal feed is, therefore, a predictable risk. Various Fusarium, Penicillium and Aspergillus...
  3. Barley cultivar identification by acid Polyacrylamide gel electrophoresis of hordein proteins

    Barley cultivar identification by acid Polyacrylamide gel electrophoresis of hordein proteins

    Item type: Journal Article • Journal: South African Journal of Plant and Soil
    Methods are described for the extraction and separation of hordein proteins of barley by acid Polyacrylamide gel electrophoresis as a means of identifying different cultivars. Experimental conditions for the extraction of the proteins from single kernels and from corresponding flour...
  4. Wheat cultivar identification by electrophoretic analysis of gliadin proteins

    Wheat cultivar identification by electrophoretic analysis of gliadin proteins

    Item type: Journal Article • Journal: South African Journal of Plant and Soil
    Polyacrylamide gel electrophoresis of gliadin proteins in an acid medium was used to identify different spring wheat cultivars. Gliadins were extracted from single kernels or corresponding flour samples using different solvents. Gliadin electrophoretogram formulae using relative band mobilities and staining...
  5. Identification of tall fescue grass cultivars by sodium dodecyl sulphate and acid polyacrylamide gel electrophoresis

    Identification of tall fescue grass cultivars by sodium dodecyl sulphate and acid polyacrylamide gel electrophoresis

    Item type: Journal Article • Journal: South African Journal of Plant and Soil
    Protein banding patterns of seed obtained by sodium dodecyl sulphate Polyacrylamide gel electrophoresis (SDS-PAGE) or acid Polyacrylamide gel electrophoresis (A-PAGE) were used to distinguish between different tall fescue (Festuca arundinacea Shreb.) grass cultivars. An electrophoretic formula was determined for each...
  6. Analysis of the HMW glutenin subunit composition of wheat cultivare by SDS-PAGE

    Analysis of the HMW glutenin subunit composition of wheat cultivare by SDS-PAGE

    Item type: Journal Article • Journal: South African Journal of Plant and Soil
    The composition of the high-molecular weight (HMW) glutenin subunits of 13 wheat cultivars grown in the Western Cape was determined using Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE). The molecular weight of the different subunits was...
  7. Identification of <em>Cyclopia</em> species (‘Heuningbostee’) using Polyacrylamide gel electrophoresis

    Identification of <em>Cyclopia</em> species (‘Heuningbostee’) using Polyacrylamide gel electrophoresis

    Item type: Journal Article • Journal: South African Journal of Plant and Soil
    Protein banding patterns of seed obtained by sodium dodecyl sulphate Polyacrylamide get electrophoresis (SDS-PAGE) or acid polycrylamide gel electrophoresis (A-PAGE) were used to distinguish between different Cyclopia species (‘heuningbostee’). An electrophoretic formula was determined for each species using relative band...
  8. The use of acid Polyacrylamide gel electrophoresis to determine the effect of locality of growth on the protein profiles of barley, wheat and honeybush tea

    The use of acid Polyacrylamide gel electrophoresis to determine the effect of locality of growth on the protein profiles of barley, wheat and honeybush tea

    Item type: Journal Article • Journal: South African Journal of Plant and Soil
    Acid Polyacrylamide gel electrophoresis (A-PAGE) of proteins was used to identify different spring wheat cultivare, barley cultivars and Cyclopia species. Experimental conditions for the extraction of the proteins from single kernels as well as corresponding flour samples are described. Different...
  9. The use of Simple Sequence Repeats (SSRs) to identify commercially important potato (<em>Solanum tuberosum</em> L.) cultivars in South Africa

    The use of Simple Sequence Repeats (SSRs) to identify commercially important potato (<em>Solanum tuberosum</em> L.) cultivars in South Africa

    Item type: Journal Article • Journal: South African Journal of Plant and Soil
    The morphological traits that are traditionally used to identify potato cultivars are not always reliable, especially when dealing with in vitro plants. Various molecular marker techniques have been described for cultivar identification, but the Simple Sequence Repeat (SSR) technique has...
  10. Development of a sequence characterized amplified region (SCAR) marker for the identification of the potato cultivars Astrid and Mnandi

    Development of a sequence characterized amplified region (SCAR) marker for the identification of the potato cultivars Astrid and Mnandi

    Item type: Journal Article • Journal: South African Journal of Plant and Soil
    Astrid and Mnandi are two closely related potato cultivars of commercial importance in Southern Africa. Random amplified polymorphic DNA (RAPD) markers were screened and a sequence characterized amplified region (SCAR) marker was developed to distinguish between these two cultivars since...