Research Note

Effect of Anti-Fungal Agents on In Vitro Cultures of Eucalyptus grandis

Published in: South African Forestry Journal
Volume 175, issue 1, 1996 , pages: 23–27
DOI: 10.1080/00382167.1996.9629889
Author(s): M.P. WattDepartment of Biology, South Africa, B.A. GauntlettDepartment of Biology, South Africa, F.C. BlakewayDepartment of Biology, South Africa


Young, small shoot explants of E. grandis were multiplied in the presence of 0,5 and 1 g/ℓ benomyl, 0,25 and 0,5 g/ℓ chlorothalonil, 0,36 and 0,72 g/ℓ propamocarb hydrochloride and 0,002 5 g/ℓ Amphotericin B. The multiplied shoots were then regenerated into plantlets in fungicide-free medium and hardened-off. At the concentrations tested, benomyl and chlorothalonil significantly inhibited survival, multiplication and growth, and the phytotoxic effects persisted after transfer to soil. At 0,36 g/ℓ, propamocarb hydrochloride did not significantly affect the total yield and development of plants, and Amphotericin B exhibited an hormone-like positive effect on growth. On the basis of these results and on the broad range anti-fungal activity of propamocarb hydrochloride, this fungicide appears to be a good agent for the eradication of fungal infections in eucalyptus cultures.

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