Scientific Paper

Establishment of Cell Suspension Cultures of Eucalyptus grandis and E. grandis x camaldulensis

Published in: South African Forestry Journal
Volume 166, issue 1, 1993 , pages: 17–26
DOI: 10.1080/00382167.1993.9629395
Author(s): F.C. BlakewayDepartment of Biology, South Africa, B. HermanMondi Forest,, M.P. WattDepartment of Biology, South Africa

Abstract

A protocol was devised for the induction and maintenance of cell suspension cultures of Eucalyptus grandis. This involved the microscopical categorisation of the various regions within heterogenous callus masses, the assessment of competence of each callus type in a liquid environment, and the determination of a medium suitable for growth of single cells. The established method allowed for the production of cell suspension cultures with a doubling time of nine to twelve days. Moreover, the protocol was applied successfully to cells of E. grandis x camaldulensis.

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