Original Articles

Development of a doubled haploid mapping population and linkage map for the bread wheat cross Kariega × Avocet S

Published in: South African Journal of Plant and Soil
Volume 22, issue 1, 2005 , pages: 1–8
DOI: 10.1080/02571862.2005.10634672
Author(s): R. Prins, South Africa, V.P. Ramburan, South Africa, Z.A. PretoriusDepartment of Plant Sciences, South Africa, L.A. BoydDepartment of Disease and Stress Biology, John Innes Centre, UK, W., H.P. Boshoff, South Africa, P.H. SmithDepartment of Disease and Stress Biology, John Innes Centre, UK, J.H. LouwDepartment of Genetics, South Africa

Abstract

A doubled haploid wheat population of 150 lines was constructed from the F1 of a cross between cultivars Kariega and Avocet S using the wheat-maize technique. Segregation and linkage analysis of 203 DNA markers, two storage protein markers and the stem rust resistance gene Sr26 yielded 31 linkage groups of which 28 were chromosome-anchored. These linkage groups covered the entire genome of 21 chromosomes. SSR markers were advantageous for chromosome anchoring and AFLP markers for consolidation of the various linkage groups. A significant feature of the linkage map is a relatively low level of polymorphism for markers on the D genome, viz, 18.5% of all markers mapped. The Kariega × Avocet S doubled haploid population and linkage maps have emerged as valuable resources for further genetic studies of qualitative and quantitative traits of economic importance in bread wheat.

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