AFLP and RAPD markers linked to leaf rust resistance gene Lr41 in wheat

Abstract

RAPD (random amplified polymorphic DNA) and AFLP (amplified fragment length polymorphism) analyses were used to identify molecular markers linked to the leaf rust resistance gene Lr41. Bulk and parental lines were screened with 380 RAPD primers and 64 AFLP primer combinations. A total of 3190 RAPD and 1859 AFLP loci were screened for linkage to the resistance gene. Twelve fragments, seven RAPD and five AFLP, were identified as putative markers for the leaf rust resistance gene Lr41. Nine of these were linked in coupling phase and three in repulsion phase. Segregation analysis of these markers in a BC₂F₂ population, segregating for the resistance gene, revealed varying linkage distances that ranged from 2.8 to 29 cM. The coupling phase AFLP marker E-AAG:M-CTA300c was converted into a sequence characterized amplified region (SCAR) marker.