Short Communications

Optimisation of transient transformation of a South African spring wheat cultivar with particle bombardment

Published in: South African Journal of Plant and Soil
Volume 17, issue 1, 2000 , pages: 50–53
DOI: 10.1080/02571862.2000.10634865
Author(s): S.M. de Villiers, Republic of South Africa, Y. LaibCentre de Developement des Techniques Nuclear, Algeria

Abstract

Transient transformation was optimised for bread wheat, Triticum aestivum L. cv Kariega, using a particle inflow gun constructed at ARC-Roodeplaat. Excised immature embryos, cultured in vitro for 5 to 7 days, were bombarded with pAHC25 and analysed for the presence of the β-glucuronidase reporter gene. Three bombardment variables were optimised and most blue spots were obtained with a helium pressure of 600 kPa, 20 mm particle flight distance and 0.4 Mosmoticum in the bombardment medium. Furthermore, the maize ubiquitin promoter was shown to be functional in Kariega.

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