Original Articles

A comparison of culture methods for tobacco (Nicotiana tabacum) anthers

Published in: South African Journal of Plant and Soil
Volume 14, issue 1, 1997 , pages: 27–30
DOI: 10.1080/02571862.1997.10635076
Author(s): AnitaE. Steyn, Republic of South Africa, J.A. Brink, Republic of South Africa, L.A. CoetzerBotany Department, Republic of South Africa

Abstract

Three different anther culture methods for the South African tobacco cultivars C11 × WOSB and CDL 28 were tested. The yield of haploid plantlets produced using a solidified or double-layer medium was significantly higher than with the use of a liquid culture medium. The former two media showed no significant differences. Important considerations which must be taken into account when deciding on the optimum culture method for anther cultures are also discussed.

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